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Research Group Stem Cells at the Interface of
hematopoiesis, Immunity and Cancer

Simon Haas
Junior Group Leader

  • MutaSeq: Single-cell analysis of leukemic stem cells - Cooperation with Velten and Steinmetz Lab published in Nature Communications

    A new method called MutaSeq allows stem cells and cancer stem cells to be studied at the single cell level and the resulting cell clones to be traced directly. The method was developed by scientists from HI-STEM, the German Cancer Research Center (DKFZ), the European Molecular Biology Laboratory (EMBL) and the Center for Genome Regulation in Barcelona. Studying thousands of individual cells in parallel, the researchers combined the analysis of the genomic cancer mutations with the associated expression profiles.

    Further Reading:

    • Publication: Velten L, Story BA, Hernández-Malmierca P, Raffel S, Leonce DR, Milbank J, Paulsen M, Demir A, Szu-Tu C, Frömel R, Lutz C, Nowak D, Jann JC, Pabst C, Boch T, Hofmann WK, Müller-Tidow C, Trumpp A, Haas S, Steinmetz LM. Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics. Nature Communication. 2021 Mar 1;12(1):1366. doi: 10.1038/s41467-021-21650-1. PMID: 33649320; PMCID: PMC7921413.
    • DKFZ Press Release
  • LeukoSystem: New BMBF-funded eMed Junior Consortium to investigate leukaemia causes and therapies

    The LeukoSyStem consortium, which is coordinated by HI-STEM group leader Simon Haas, investigates leukaemia stem cells in acute myeloid leukaemia (AML). The German Federal Ministry of Education and Research (BMBF) financially supports this collaboration with a total sum of €2.45 M for five years as part of its eMED program on systems medicine. LeukoSystem is a collaboration between junior researchers, namely Dr. Simon Raffel (Heidelberg University Hospital), Lars Velten (CRG Barcelona), Laleh Haghverdi (EMBL Heidelberg) and Simon Haas (HI-STEM gGmbH and German Cancer Research Center (DKFZ)).

    The aim of their project is to investigate the cells that are the origin of acute myeloid leukaemia (AML) and thus "get to the root of the problem". The scientists intend to use isolated single cells from patient samples to investigate characteristic markers, mutations, functional data, and metabolic pathways, to gain a better understanding of leukaemia stem cells and their environment in the bone marrow. The collected data will be evaluated comprehensively with the help of computer algorithms specially developed for the purpose.


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  • New paper out on Nature Cell Biology: 3D atlas of the bone marrow - in single cell resolution

    Three dimensional segmentation of a bone marrow region. Different niche cells (green and red dots) and blood vessels (grey) are highlighted. | © DKFZ, EMBL and University Hospital Zurich

    The lastest paper from the group of Simon Haas was published by Nature Cell Biology shortly before Christmas last year. This is the result of a great team effort together with colleagues at the DKFZ and HI-STEM.

    The published methods are able to reveal the three-dimensional organization of the bone marrow at the single cell level. Using this approach the teams have identified previously unknown cell types that create specific local environments required for blood generation from stem cells. The study reveals an unexpected complexity of the bone marrow and its microdomains at an unprecedented resolution and provides a novel scientific basis to study blood diseases such as leukemias.

    Read more:

    • Original Paper: Baccin, C.*, Al-Sabah*, J., Velten, L.*@, Helbling, P. M., Grunschlager, F., Hernandez-Malmierca, P., Nombela-Arrieta, C., Steinmetz, L. M.@, Trumpp, A.@, & Haas, S.@ (2019). Combined single-cell and spatial transcriptomics reveal the molecular, cellular and spatial bone marrow niche organization. Nature Cell Biology. doi: 10.1038/s41556-019-0439-6*: Shared first authors, @ Joint Supervising authors
    • News and Views Article: Lucas, D., Salomonis, N., & Grimes, H. L. (2019). Unraveling bone marrow architecture. Nat Cell Biol. doi: 10.1038/s41556-019-0447-6
    • DKFZ Press Release
    • EMBL Press Release
  • New Review in Cell Stem Cell: Causes and Consequences of Hematopoietic Stem Cell Heterogeneity

    Classically, stem and progenitor populations have been considered discrete homogeneous populations. However, recent technological advances have revealed significant hematopoietic stem cell (HSC) heterogeneity, with evidence for early HSC lineage segregation and the presence of lineage-biased HSCs and lineage-restricted progenitors within the HSC compartment. These and other findings challenge many aspects of the classical view of HSC biology.

    In our recent review in Cell Stem Cell, we analyse the most recent findings regarding the causes and consequences of HSC heterogeneity, discuss their far-reaching implications, and suggest that so-called continuum-based models may help consolidate apparently divergent experimental observations in this field.

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  • Double sucess for HI-STEM researchers Simon Haas and Simon Raffel

    Two awards at once, both carrying high monetary prizes, go to two HI-STEM members: Simon Raffel will receive the 2017 Walter Schulz Prize for his discovery how misregulated breakdown of amino acids in leukemia stem cells promotes blood cancer. To find out more about this work, read our news feature.

    Simon Haas will share the 2018 Otto Schmeil Prize with his colleague Lars Velten from EMBL. The two stem cell researchers have jointly demonstrated that the development of blood cells in the bone marrow follows very different paths from what scientists have assumed up to now.

     

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  • Travel Grant to Simon Haas

    The Boehringer Ingelheim Foundation has awarded a travel grant to Simon Haas for visiting research at Harvard Medical School.

  • New study published in Nature Cell Biology

    Human haematopoietic stem cell lineage commitment is a continuous process (Nature Cell Biology, 2017)

    Velten, L.*, Haas, S.F.*, Raffel, S.*, Blaszkiewicz, S., Islam, S., Hennig, B.P., Hirche, C., Lutz, C., Buss, E.C., Nowak, D., Boch, T., Hofmann, W.K., Ho, A.D., Huber, W., Trumpp, A.@, Essers, M.A.@, & Steinmetz, L.M.@ (2017). Human haematopoietic stem cell lineage commitment is a continuous process. Nature Cell Biology, 19(4), 271-281. doi: 10.1038/ncb3493 Cover Story *Shared first author @Shared corresponding author